UAB Comprehensive Center for Healthy Aging
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Cell Senescence Culture Facility

Sponsors:
UAB Comprehensive Center for Healthy Aging; UAB Department of Biology

Purpose:
The general purpose of the Cell Senescence Culture Facility (CSCF) is to provide various types of aging cells to investigators interested in the aging process. This facility is one of only a few in the United States and is designed to not only facilitate studies of aging, but to also participate in new investigations of the mechanisms of cellular aging and age-related diseases. The CSCF is available to investigators who are actively involved in studies of cellular aging as well as those who are considering aging studies. It is intended to facilitate understanding of the basis of aging and to encourage study of age-related diseases using in vitro models of senescent cell lines. A prime goal of the CSCF is to develop a research focus that impinges on the understanding of the basic phenotypic changes in senescent cells as well as the prevention of senescent pathways.

Services:
The services provided by the CSCF include:

  1. Growing and supplying various cell lines to investigators who have an interest in cell senescence or age-related diseases such as Alzheimer's disease, osteoporosis and bone and metabolic diseases, degenerative joint disease and cancer as well as many other age-related conditions.
  2. Collecting and maintaining an aging cell line repository for storage of cell lines that provides ready access of cells to all interested investigators.
  3. Conducting research into the fundamental and clinical aspects of cell senescence using specific cell lines to probe the cell culture characteristics of phenotypic changes and underlying molecular or metabolic changes.
  4. Performing a consultation service in growing various age-related cell lines for experimental projects. We have the ability to record cell morphology in culture conditions using our Nikon triocular phase contrast light microscope and Nikon 990 digital camera. The facility has several incubators that can be used to control culture conditions for special studies. We can passage cells to a certain population doubling or to senescence by morphological standards. We can store cells in liquid nitrogen and provide frozen cell pellets for protein or DNA purification. The facility also provides services for immunohistochemistry analysis using specified antibodies, examination of telomerase activity and analysis of biomarkers of aging such as the beta-galactosidase assay.

Opportunities for Faculty Involvement:
All UAB faculty are eligible to participate in the use of the CSCF. Besides basic science faculty, we are also very interested in the involvement of clinical faculty who study diseases related to the aging process.

Primary Contact Person:

, Ph.D., D.O., Assistant Professor
Department of Biology
175A Campbell Hall
1300 University Boulevard
University of Alabama at Birmingham
Birmingham, AL 35294-1170
(tel) (205) 934-4573
(fax) (205) 975-6097

Web Sites:
All UAB faculty are eligible to participate in the use of the CSCF. Besides basic science faculty, we are also very interested in the involvement of clinical faculty who study diseases related to the aging process.

Leaders and Key Staff:

  • Trygve Tollefsbol, D.O., Ph.D., Director

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